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2 edition of study of the binding of oligosaccharides and glycopeptides to concanavalin A. found in the catalog.

study of the binding of oligosaccharides and glycopeptides to concanavalin A.

Alexander Euan MacKenzie

study of the binding of oligosaccharides and glycopeptides to concanavalin A.

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Published .
Written in English


The Physical Object
Pagination[213] leaves
Number of Pages213
ID Numbers
Open LibraryOL14762788M

  Using molecular dynamics (MD) simulation methods, we attempted to explain the experimental results on ligand specificity of glucose/galactose-binding protein (GGBP) to β-D-glucose and by: 2. Binding of DNA oligonucleotides to sequences in the promoter of the human bcl-2 gene Wendy M. Olivas+ and L. James Maher, III1,* Eppley Institute for Research in Cancer and Allied Diseases and the Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, S. 42nd Street, Omaha, NE , USA and. Desialation of glycopeptides was carried out by incubating with 5 U of neuraminidase in 20 mM Tris-HCl buffer, pH at 37 °C for 4 h, the enzyme and sialic acid were removed by successive chromatography on Sephadex G ( × 10 cm) and Dowex as described above. Fluorescence measurements and ligand-binding data analysis. Whether you've loved the book or not, if you give your honest and detailed thoughts then people will find new books that are right for them. 1 Carbohydrate chemistry: monosaccharides, disaccharides, and specific oligosaccharides, A review of the literature published in


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study of the binding of oligosaccharides and glycopeptides to concanavalin A. by Alexander Euan MacKenzie Download PDF EPUB FB2

Concanavalin A is a tetrameric protein with four identical 28 kDa subunits. 29 The minimal structure exhibiting high affinity binding of carbohydrates is the dimeric form. The dimer form of ConA is an active stable structure, and the tetramer–dimer transition can be promoted by low pH or succinylation of the protein.

30 For reasons of economy. We have investigated the binding of a series of high affinity asparagine-linked glycopeptides, including high mannose type and a bisected hybrid type, and several related synthetic oligosaccharides, to Ca2+- Mn2+-concanavalin A (ConA), using solvent proton nuclear relaxation dispersion (NMRD) by: We have recently demonstrated that certain oligomannose and bisected hybrid-type glycopeptides are bivalent for concanavalin A (ConA) binding and that they can precipitate the lectin.

The structural requirements for the binding of oligosaccharides and glycopeptides to immobilized Vicia faba agglutinin were investigated, and its carbohydrate specificity was compared with the. Lectins are proteins with sugar binding activity. Each lectin binds specifically to a certain sugar sequence in oligosaccharides and glycopeptides.

To overcome these problems, lectins can serve as very useful tools. Recently, many attempts have been made to fractionate oligosaccharides and glycopeptides on immobilized lectin by: 9. Narasimhan, S., Freed, J. C., and Schachter, H.,The effect of a “bisecting” N-acetylglucosaminyl group in the binding of biantennary, complex oligosaccharides to concanavalin A, Phaseolus vulgaris erythroagglutinin (E-PHA), and Ricinus communis agglutinin (RCA) immobilized on Cited by: 9.

1. Angew Chem Int Ed Engl. May 4;54(19) doi: /anie Epub Mar Glycopeptide mimetics recapitulate Cited by: 3. A class of new glycan-reactive broadly neutralizing antibodies represented by PGT, 10–, and PGT has recently been discovered that targets specific N-glycans and the peptide region around the V3 domain.

However, the glycan specificity and fine epitopes of these bNAbs remain to be further defined. We report here a systematic chemoenzymatic synthesis of homogeneous V3 Cited by: 8. Glycopeptides. Sussex Research prepares a wide variety of custom glycopeptides. Sussex has years of experience in the glycopeptide field.

We welcome your custom synthesis and will adapt our synthetic methodologies to accommodate your specific needs. Please consult with us on your project requirements. @article{osti_, title = {O-linked oligosaccharides on insulin receptor}, author = {Collier, E and Gorden, P}, abstractNote = {The insulin receptor, an integral membrane glycoprotein, is synthesized as a single-chain precursor that is cleaved to produce two mature subunits, both of which contain N-linked oligosaccharide chains and covalently linked fatty acids.

Glycopeptides; Glycopeptides. In the synthesis of the glycosylated compounds such as glycopeptides and Maillard reaction products, a high level of carbohydrate chemistry knowledge and experience are vital to.

The binding of fluorescently labelled carbohydrates to concanavalin A and wheat germ agglutinin was studied at equilibrium and by the stopped-flow and temperature jump relaxation methods.

Ligand were mainly die 4-methylumbelliferyl glycosides of alpha (1 → 2)-linked mannooligosaccharides and of beta (1 → 4)-linked chitooligosaccharides as limited homologous series. 2 metal ion binding sites. Metal ions (Ca2+ and Mn2+) must be bound to these sites in order for the sugar binding to occur.

This Concanavalin A product is highly purified and aseptically filled. It is lyophilized powder from a solution containing buffer salts and NaCl. This Con A File Size: 80KB. Microheterogeneity in Oligosaccharides.- Class A Glycopeptides.- Changes in Oligosaccharides Derived from Single Glycoproteins.- 4.

Changes in Serine- (Threonine-) Linked Oligosaccharides of Membrane Glycoproteins.- 5. Alterations in Glycosaminoglycans.- 6. Use of Endoglycosidases for Detecting Alterations in Cell Surface. wall glycopeptides and the oligosaccharides derived from them consist of alternating units of GlcNAcl and its lactyl ether, N-acetylmuramic acid.

Recently, several groups have deter- mined binding constants with lysozyme for a number of oligomers of GlcNAc: Lehrer and Fasman (6) studied the binding of. COVID Resources. Reliable information about the coronavirus (COVID) is available from the World Health Organization (current situation, international travel).Numerous and frequently-updated resource results are available from this ’s WebJunction has pulled together information and resources to assist library staff as they consider how to handle coronavirus.

In this study, DMS-MS was employed for the structural analysis of glycans and glycopeptides. The separation of the isomeric glycan and glycopeptide species studied here was accomplished because of differences in the DMS behavior between each isomer9.

The ions bear subtle structural differences that allow the DMS to separate them basedFile Size: KB. Glycosidic Bond Expanded Cyclic Oligosaccharides: Synthesis and Host−Guest Binding Property of a Cyclic Pentasaccharide Gopal Ch Samanta, Krishnagopal Maiti, and Narayanaswamy Jayaraman* Department of Organic Chemistry, Indian Institute of Science, BangaloreIndia * S Supporting Information ABSTRACT: A new cyclic pentasaccharide.

The crystal structure of Pterocarpus angolensis seed lectin is presented in complex with a series of high mannose (Man) oligosaccharides ranging from Man-5 to Man Despite that several of the nine Man residues of Man-9 have the potential to bind in the monosaccharide-binding site, all oligomannoses are bound in the same unique way, employing the tetrasaccharide sequence Man α Cited by:   Oligosaccharides Linked through N-Acetylgalactosamine to Serine and Threonine.- Oligosaccharides Linked to Serine and Threonine through Sugars Other Than N-Acetylgalactosamine.- Oligosaccharides Linked to the Hydroxyl Group of Hydroxylysine and Hydroxyproline.- 4.

Glycopeptides Containing the N-Acetylglucosaminyl-Asparagine Linkage.- Author: William Lennarz. C.A.S.: First crystallized by Sumner and Howell (), Concanavalin A (ConA) has proven to be an interesting and useful lectin.

Lectins are proteins that react with specific terminal sugar residues and are useful probes in studying carbohydrates of cell surfaces. The composition of N-linked glycoprotein oligosaccharides was studied in myoblasts and myotubes of the C2 muscle cell line.

Oligosaccharides were radioactively labelled for 15 hr with (TH) mannose and plasma membranes isolated. Ten glycopeptides were detected by SDS-PAGE and fluorography. Conjugation of oligosaccharides to probes and surfaces is useful in the development of biochemical assays to assess carbohydrate–protein interactions.

Cited by: 3. Glycosylation analysis based on mass spectrometry (MS) of glycopeptides requires the isolation of glycopeptides from complex glycoprotein digests to facilitate structural determin.

Among them, N-linked glycopeptides are the most common type in nature, with N referring to the asparagine that attached by the glycan. Glycan of O-linked glycopeptides is commonly linked to the hydroxyl groups of serine and threonine, while sometimes also binding to lysine, proline and tyrosine.

study we could not saturate the protein sample with maltopen-taose, maltotetraose, maltotriose and glucosyl-maltotriose. However, all oligosaccharides that induced chemical shift changes in b1-CBM showed similar patterns of change upon ligand binding suggesting a single carbohydrate-binding site (Figs.

1B and C, 2 and 2S, Supplementary data). Glycopeptides and Glycoproteins: Synthesis, Structure, and Application (Topics in Current Chemistry ()) [Wittmann, Valentin] on *FREE* shipping on qualifying offers. Glycopeptides and Glycoproteins: Synthesis, Structure, and Application (Topics in Current Chemistry ())Format: Hardcover.

Ovalbumin Glycopeptides*t RICHARD H. NUENKE~ AND LEON W. CUNNINGHAM From the Department of Biochemistry, School of Medicine, Vanderbilt University, Nashville 5, Tennessee (Received for publication, Ma ) The first clear demonstration of the glycoprotein nature of ovalbumin was made by Neuberger in (2).

A Calorimetric Study of the Interaction of Oligosaccharides and Hen Ovalbumin Glycopeptides with Concanavalin A, BiochemisAnsari, A., Ahmad, R., and Salahuddin, A.: The Native and Denatured States of Ovalbumin, Biochem J, The characterization of the binding of reducing carbohydrates present as mixtures of anomers in solution to a sugar recepor (lectin) poses severe difficulties.

In this situation, NMR spectroscopy enables the observation of signals for each anomer in the mixture by applying approaches based on ligand observation. Saturation transfer difference (STD) NMR allows fast and efficient screening of Cited by: The binding of glycopeptides from porcine thyroglobulin with a high variety of complex N-glycans showed that some of these glycopeptides were retarded by the immobilized tepary bean lectin.

The chromatogram depicted in Figure 5 shows three distinct fractions: fraction 1 corresponds to the unbound (run-through) material, and fractions 2 and 3 Cited by: The resulting glycopeptides are generally suitable substrates for Peptide:N‐glycosidase F, an enzyme useful in defining oligosaccharide‐peptide linkages.

Additionally, they can be separated by reversed‐phase chromatography, resulting in a glycopeptide map that is analogous to a peptide map, and used for detection of glycosylation : Leland D. Powell. The binding of fluorescently labelled carbohydrates to concanavalin A and wheat germ agglutinin was studied at equilibrium and by the stopped-flow and temperature jump relaxation methods.

Ligand were mainly die 4-methylumbelliferyl glycosides of α (1 → 2)-linked manno-oligosaccharides and of β (1 → 4)-linked chito oligosaccharides as. CHAPTER 1. Introduction. BY J. KENNEDY. The Introduction to the Part II Report was revised extensively in Volume That Chapter, and the additions in Vol covered in detail: 'Scope and Coverage of the Report'; 'Organization, Nomenclature, and Use of the Report'; 'Significant Advances in Macromolecular Carbohydrate Chemistry'; and 'Conclusions and Readership', is classified,as Pages: Synthesis of Glycopeptides and Lipopeptides by Chemoselective Ligation* Dipl.‐Chem.

Stefano E. Cervigni. addressable groups react under very mild conditions with free reducing sugars or lipidic aldehydes to form glycopeptides or On-bead synthesis and binding assay of chemoselectively template-assembled multivalent.

especially its versatility in binding different linear and cyclic oligosaccharides with similar affinities, we have undertaken high-resolution X-ray analysis of three oligosaccharide-bound structures. Results: The structures of MBP complexed with maltose, maltotriose and maltotetraose have been refined to high resolutions ( to Å).

These. The chemical synthesis of oligosaccharides is much more complicated than the synthesis of other biopolymers such as peptides and oligonucleotides and many challenging problems still need to be addressed.

Some projects uses synthetically modified saccharides to study the factors that are important for carbohydrate-protein complexation and it. Mannosyl glycolipids with perfluoroalkyl membrane anchors have been synthesised. When inserted into vesicles, these mannosyl lipids either dispersed evenly over the surface or, in the presence of cholesterol, phase-separated into artificial lipid rafts.

At 1% mol/mol, the affinity of dispersed mannosyl lipidCited by: An expedient and simple protocol to access S-linked glycopeptides by Fmoc SPPS using unprotected carbohydrates is reported.

The utility of the method was demonstrated with the solid phase synthesis of a MUC1 fragment (20 mer) containing two glycosylation sites that were substituted with S-linked by: Molecular architecture with carbohydrate functionalized β-peptides adopting helical conformation Nitin€J.€Pawar1,2, Navdeep€S.€Sidhu3, George€M.€Sheldrick3, Dilip€D.€Dhavale*2 and€Ulf€Diederichsen*1 Full Research Paper Open Access Address: 1Institute for Organic and Biomolecular Chemistry, Georg-August University Göttingen, Tammannstrasse 2, D Göttingen,Cited by: 3.

Molecular cloning of the “old” but still unclassified Euonymus europaeus agglutinin (EEA) demonstrated that the lectin is a homodimeric protein composed of residue subunits.

Analysis of the deduced sequence indicated that EEA is synthesized without a signal peptide and undergoes no posttranslational processing apart from the removal of a six-residue N-terminal by: Study Oakland Community College Biology flashcards and notes.

Conquer your course and sign up for free today!Here we report the LC–MS/MS quantitative analyses of human blood serum glycoproteins and glycopeptides associated with esophageal diseases by LAC- and HC-based enrichment.

The separate and complementary qualitative and quantitative data analyses of protein glycosylation were performed using both enrichment by: